Genome Editing of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells With Zinc Finger Nuclease for Cellular Imaging

Rationale—Molecular imaging has proven to be a vital tool in the characterization of stem cell behavior in vivo. However, the integration of reporter genes has typically relied upon random integration, a method that is associated with unwanted insertional mutagenesis and position effects on transgene expression. Objective—To address this barrier, we used genome editing with zinc finger nuclease technology to integrate reporter genes into a safe harbor gene locus (PPP1R12C, also known as Address correspondence to: Dr. Joseph C. Wu, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Room G1120B, Stanford, CA 94305-5111, Tel: 650-736-2246, Fax: 650-736-0234, [email protected]. *Y.W and W.Y.Z contributed equally to this study. DISCLOSURES FDU is a full-time employee of Sangamo Biosciences, Inc. Subject codes: [139] Developmental biology [142] Gene expression [89] Genetics of cardiovascular disease [150] Imaging [124] Cardiovascular imaging agents/Techniques This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHS Public Access Author manuscript Circ Res. Author manuscript; available in PMC 2013 December 07. Published in final edited form as: Circ Res. 2012 December 7; 111(12): 1494–1503. doi:10.1161/CIRCRESAHA.112.274969. A uhor M anscript